RESUMO
ABSTRACT Objectives This study aimed to evaluate the frequencies of the angiotensin converting enzyme (ACE) gene insertion/deletion (I/D) and methylenetetrahydrofolate reductase (MTHFR) gene C677T polymorphisms in obese patients with and without type 2 diabetes mellitus (T2DM). Subjects and methods These polymorphisms were analyzed by polymerase chain reaction in 125 patients with obesity, 47 (T2DM) and 78 (Control Group). Results No significant difference was found on comparing the T2DM and Control Groups in respect to the genotypic frequencies of the polymorphisms - (II: 13.3% vs. 12.0%; ID: 37.8% vs. 37.3; DD: 48.9% vs. 50.7%; CC: 36.2% vs. 39.0%; CT: 46.8% vs. 49.3%; TT: 17.0% vs. 11.7%), and alleles (I: 32.2% vs. 30.7%; D: 67.8% vs. 69.3%; C: 59.6% vs. 63.6%; T: 40.4% vs. 36.4%) and their synergisms in the pathophysiology of T2DM. On analyzing the T2DM Group, there were no significant differences in the presence of complications. In this population of Brazilian obese patients, no correlation was found between the ACE and MTHFR polymorphisms in the development of T2DM. Conclusion Analyzing only the group with diabetes, there was also no relationship between these polymorphisms and comorbidities.
Assuntos
Humanos , Masculino , Feminino , Adolescente , Adulto , Pessoa de Meia-Idade , Idoso , Adulto Jovem , Polimorfismo Genético/genética , Peptidil Dipeptidase A/genética , Metilenotetra-Hidrofolato Redutase (NADPH2)/genética , Diabetes Mellitus Tipo 2/enzimologia , Obesidade/complicações , Brasil , Estudos de Casos e Controles , Reação em Cadeia da Polimerase , Fatores de Risco , Mutagênese Insercional , Deleção de Genes , Predisposição Genética para Doença , Diabetes Mellitus Tipo 2/complicações , Diabetes Mellitus Tipo 2/genética , Genótipo , Obesidade/enzimologiaRESUMO
La diabetes mellitus tipo 2 es una de las epidemias de mayor impacto a nivel mundial. La susceptibilidad genética constituye un importante factor de riesgo en ella, que se adiciona al efecto de los factores ambientales y al modo de vida. Explorar el papel del polimorfismo Gly1057Asp del gen IRS-2 en la susceptibilidad genética para la diabetes mellitus tipo 2. Se estudió la frecuencia del polimorfismo Gly1057Asp del IRS-2 en 499 ciudadanos cubanos, con IMC³22 y <30, con edades comprendidas entre los 40 y 70 años, de ellos 272 (54,5 por ciento) diabéticos y 227 (45,5 pro ciento) no diabéticos. Para la comparación de las frecuencias de este polimorfismo en ambos grupos utilizamos la prueba chi cuadrado (p<0,05) y para la cuantificación de las asociaciones se utilizó la razón de productos cruzados. La frecuencia del alelo Asp1057 del polimorfismo Gly1057Asp del gen IRS-2 fue de 49,8 por ciento en el grupo de pacientes diabéticos, y de 58,8 por ciento en el grupo de los no diabéticos (p<0,05), lo cual sugiere que este alelo confiere protección contra la diabetes. Sin embargo, este patrón de distribución de frecuencias cambia en personas con sobrepeso, en las que la presencia del alelo Asp1057 llega a asociarse al hiperinsulinismo. En sujetos con antecedentes paternos y/o maternos de diabetes mellitus tipo 2 la acción protectora del alelo Asp1057 no se expresa. En el grupo de la población cubana estudiada el alelo Asp1057 del gen IRS-2 confiere protección para la diabetes mellitus tipo 2. El sobrepeso y los antecedentes maternos y/o paternos de diabetes modifican esta acción protectora(AU)
Type 2 diabetes mellitus is one of the more hard-bitting pandemic at world level. Genetic susceptibility is an important risk factor of it, as well as the effect of environmental factors, and lifestyles. to explore role of Gly1057Asp polymorphism of IRS-2 gene in genetic oversensitivity for Type 2 diabetes mellitus. Frequency of Gly1057Asp polymorphism of IRS-2 gene in was studied in 499 Cuban citizens, with IMC³22 and <30, aged from 40 to 70, of them, 272 (54,5 percent) were diabetic people and 277 (45,5 percent) were non-diabetic. For comparison of frequency of this polymorphism in both groups, we used 2Chi test (p<0,05), and to quantify associations, we used crossed-products ratio. Frequency of Asp1057 allele of Gly1057Asp polymorphism of IRS-2 gene was of 49,8 percent in diabetic patient group, and of 58,8 percent in the group on non-diabetic one (p<0,05), suggesting that this allele confers protection against diabetes. However, this pattern of frequency distribution changes in persons overweighed, in which presence of Asp1057 allele becomes associated with hyperinsulinism. In subjects with family backgrounds of Type 2 diabetes mellitus, protection of Asp1057 allele is not expressed. In group of study Cuban population, Asp1057 allele of IRS-2 gene confers protection against Type 2 diabetes mellitus. Overweight and family backgrounds modify this protective action(AU)